Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160949_ghg0 ACTTCCTCAGGTCTCCACTTCTCTTGCACCAG(+AAAAAA)GATCCACCACACCCAGTCTTATCACGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240606160948_fz76 ATTCCTAGCACGTGATAAGACTGGGTGTGGTG(+AAAAAA)GATCCTGGTGCAAGAGAAGTGGAGACCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606160947_qq9t AAAAAGTCCCCTCAGACTCTTTATTCACTTAG(+AAAAAA)GATCCTTGCGAGCCATTGGACTTGACTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606160946_67rq TGCCTGTGAAGTCAAGTCCAATGGCTCGCAAG(+AAAAAA)GATCCTAAGTGAATAAAGAGTCTGAGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606160944_8get GCTTGATATGCAAGCATGAGGAACTGAGTTCG(+AAAAAA)GATCCCCAGAGCCCATGTAAGAGCCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606160942_tfqh ACTAGGAGCCTGGCTCTTACATGGGCTCTGGG(+AAAAAA)GATCCGAACTCAGTTCCTCATGCTTGCATAT NGG 10 8 17 8 24 0 100 5 10
    240606160940_cb5n ATGGTATCTCACAACCATCCATACATCCAGGG(+AAAAAA)GATCCGATGCCTCCTTCTGACCTCCAAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606160938_ml8m CACATGCTCTTGGAGGTCAGAAGGAGGCATCG(+AAAAAA)GATCCCCTGGATGTATGGATGGTTGTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606160937_j8dm TAGCAGCCACATAAGCTGGGCAAGCTGAGTTG(+AAAAAA)GATCCCCAGAACCCAGGTAATCAGCCAGATA NGG 10 8 17 8 24 0 100 5 10
    240606160935_quk4 CGAGATATCTGGCTGATTACCTGGGTTCTGGG(+AAAAAA)GATCCAACTCAGCTTGCCCAGCTTATGTGGC NGG 10 8 17 8 24 0 100 5 10