Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160907_t0zz TCCTGTATCTTGCCTTTGTACAAGAGCATATG(+AAAAAA)GTACCTTTTTGAATAAAATAACATTTAACAT NGG 10 8 17 8 24 0 100 5 10
    240606160906_xdd7 TAAATATGTTAAATGTTATTTTATTCAAAAAG(+AAAAAA)GTACCATATGCTCTTGTACAAAGGCAAGATA NGG 10 8 17 8 24 0 100 5 10
    240606160904_4ok7 AGGAAGCTCTGGCAATTCTGGTTCTTAACCAG(+AAAAAA)GATCCAGGAGGCATCACTGGGAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240606160902_zs2i TTGAAATCACACTTTCCCAGTGATGCCTCCTG(+AAAAAA)GATCCTGGTTAAGAACCAGAATTGCCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606160901_zi47 GATCGTTGAATCTCTCTCATCCCAGTGCAATG(+AAAAAA)GATCCACAAATCCGTGGGATGCAAAAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606160900_dzkg AGTTCTCTGCTTTTGCATCCCACGGATTTGTG(+AAAAAA)GATCCATTGCACTGGGATGAGAGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240606160859_sf2k ACAAATAGCTCCCCAGGATCTGATTAAGATAG(+AAAAAA)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606160856_e0j3 AAAATGTCCTTGATAATAAATGGTATGGCCCG(+AAAAAA)GATCCTATCTTAATCAGATCCTGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606160855_hjx0 ATTTGAAATAAAGGTTTTAATTCTGAAGTAGG(+AAAAAA)GATCCTTAAATAAGGTCTAATCCAATTTATA NGG 10 8 17 8 24 0 100 5 10
    240606160854_uy2x GGAGATATAAATTGGATTAGACCTTATTTAAG(+AAAAAA)GATCCCTACTTCAGAATTAAAACCTTTATTT NGG 10 8 17 8 24 0 100 5 10