Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160921_aobp CTGAAAACATAACTTAAATAGACTGACTGGAG(+AAAAAA)GATCCGCCACACACGTGTTCATGGATGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606160919_03wm CACTGTTCCCATCCATGAACACGTGTGTGGCG(+AAAAAA)GATCCTCCAGTCAGTCTATTTAAGTTATGTT NGG 10 8 17 8 24 0 100 5 10
    240606160918_1ztt CTGGATTGGAACTCATTAAGACCAACAGCCAG(+AAAAAA)GATCCCGATTTTGAGCCTAAACTTTTGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606160916_tfrc AGGAAGGAACAAAAGTTTAGGCTCAAAATCGG(+AAAAAA)GATCCTGGCTGTTGGTCTTAATGAGTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606160915_ah7r TATAATTTATGCAGAGAAATTTCACTTACATG(+AAAAAA)GTACCTTTTCCAGTCTCAACATCTCATCATA NGG 10 8 17 8 24 0 100 5 10
    240606160914_42kv CATTTTATGATGAGATGTTGAGACTGGAAAAG(+AAAAAA)GTACCATGTAAGTGAAATTTCTCTGCATAAA NGG 10 8 17 8 24 0 100 5 10
    240606160912_hxz1 GGAAGGTTGGTCCCACAAGATGCACTGAAATG(+AAAAAA)GATCCCCACCCAATAAAGATGGTATTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240606160911_ypm0 CATATGTAACAATACCATCTTTATTGGGTGGG(+AAAAAA)GATCCATTTCAGTGCATCTTGTGGGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240606160909_o1qt TACATAAACCAATGCTTGAAGATGCTGCAAAG(+AAAAAA)GATCCTCTAGTGATTCCTGATGAGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240606160908_tm45 CTATTCTGATTCCTCATCAGGAATCACTAGAG(+AAAAAA)GATCCTTTGCAGCATCTTCAAGCATTGGTTT NGG 10 8 17 8 24 0 100 5 10