Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160852_rjz2 AGGGAACCCAGGGTCACCTGCAGGTGACAGAG(+AAAAAA)GTACCCGCCTCCACTTAATGTCCATCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606160850_dqqz ACCCCAGCTTGATGGACATTAAGTGGAGGCGG(+AAAAAA)GTACCTCTGTCACCTGCAGGTGACCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606160849_knn6 GGTGGGCTAAGTTGCCAAAAACGACAATCAGG(+AAAAAA)GATCCAATAAGCAATCTTGGTCACTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606160848_nx0y AAATTTACTTAAGTGACCAAGATTGCTTATTG(+AAAAAA)GATCCCTGATTGTCGTTTTTGGCAACTTAGC NGG 10 8 17 8 24 0 100 5 10
    240606160847_i5x1 TTCTTAGTATTCAGAATTGATGTGTTTTATTG(+AAAAAA)GTACCAGCTGTCTTAATGCATTTATTAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606160845_dj4v AACTGTTCTTAATAAATGCATTAAGACAGCTG(+AAAAAA)GTACCAATAAAACACATCAATTCTGAATACT NGG 10 8 17 8 24 0 100 5 10
    240606160844_s669 GTCTCACACCTCAGTCCCTTGGTCACACTAAG(+AAAAAA)GTACCCAAGATGTTGGCCAGGATTACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240606160842_0jbn GGAATCTCTAGTAATCCTGGCCAACATCTTGG(+AAAAAA)GTACCTTAGTGTGACCAAGGGACTGAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606160841_mclb AGTTAGCTGGGGCAGAGGTATGGTTGGAGTAG(+AAAAAA)GTACCTAGGACGGCAACCCCTTCGTAATAGG NGG 10 8 17 8 24 0 100 5 10
    240606160839_t0yv ACCCCCCTATTACGAAGGGGTTGCCGTCCTAG(+AAAAAA)GTACCTACTCCAACCATACCTCTGCCCCAGC NGG 10 8 17 8 24 0 100 5 10