Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160838_ro1e TGACAGTAACTGATCTGCATTAGTTAGGAGTG(+AAAAAA)GTACCGCTTTCATCAGTTCAATGATGCTACT NGG 10 8 17 8 24 0 100 5 10
    240606160837_s7cj TGTAAAGTAGCATCATTGAACTGATGAAAGCG(+AAAAAA)GTACCACTCCTAACTAATGCAGATCAGTTAC NGG 10 8 17 8 24 0 100 5 10
    240606160835_u4pu GAAGAACTCTTATGAATACATTAAAGTAAAAG(+AAAAAA)GATCCCTAAAACGATTAAGCATACAGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606160834_h0hs ATTGTCCAACCTGTATGCTTAATCGTTTTAGG(+AAAAAA)GATCCTTTTACTTTAATGTATTCATAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606160833_dk74 TTCTGTGCCTCAAGGAAAAGGAGCCCTCTTAG(+AAAAAA)GTACCGCCACAACATGGATGATCCAGACTGT NGG 10 8 17 8 24 0 100 5 10
    240606160831_39hn AGGCAACAGTCTGGATCATCCATGTTGTGGCG(+AAAAAA)GTACCTAAGAGGGCTCCTTTTCCTTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606160830_cu5l TTGGATCCAGTGACATAGCTAATAGGGTGAAG(+AAAAAA)GTACCTGCCACCAACTCTGATAATTTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606160828_n9qn GATTGAACTCAAATTATCAGAGTTGGTGGCAG(+AAAAAA)GTACCTTCACCCTATTAGCTATGTCACTGGA NGG 10 8 17 8 24 0 100 5 10
    240606160826_vuvy TTTTATTCCTATCAAAAATGTCTTTATGCCTG(+AAAAAA)GATCCATCATCAAATAGTGTTCAATTCACTG NGG 10 8 17 8 24 0 100 5 10
    240606160825_4wqc CAAACCAGTGAATTGAACACTATTTGATGATG(+AAAAAA)GATCCAGGCATAAAGACATTTTTGATAGGAA NGG 10 8 17 8 24 0 100 5 10