Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130726_vut0 TGCCAGTGAATTCTCTGAGGAAGCCTGCGATG(+AAAAAA)GATCCTTTCAGGTGACTTATTACATAGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606130725_9s3i GAGAGAACCCTATGTAATAAGTCACCTGAAAG(+AAAAAA)GATCCATCGCAGGCTTCCTCAGAGAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240606130724_5j77 GAAAGAGTTGGCAATTCAGACTTTCTTGTTTG(+AAAAAA)GATCCTATTATCAGCTCCTTAGGGTTTGTAT NGG 10 8 17 8 24 0 100 5 10
    240606130722_vhkx TCAAGATACAAACCCTAAGGAGCTGATAATAG(+AAAAAA)GATCCAAACAAGAAAGTCTGAATTGCCAACT NGG 10 8 17 8 24 0 100 5 10
    240606130721_naxr CCTGCAGTGTATACAAAAGAATTTACTCCTTG(+AAAAAA)GATCCCTTGTGTTTGGCTACAGTTACCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606130719_tx4n TTCCAAACAGGTAACTGTAGCCAAACACAAGG(+AAAAAA)GATCCAAGGAGTAAATTCTTTTGTATACACT NGG 10 8 17 8 24 0 100 5 10
    240606130717_meaz GGTGCCAGCCTTGGGGCACACCGCTCCACCGG(+AAAAAA)GTACCGCTGCAGCCACTGTGTTACTGTAAGC NGG 10 8 17 8 24 0 100 5 10
    240606130715_v718 GTGTGGCTTACAGTAACACAGTGGCTGCAGCG(+AAAAAA)GTACCCGGTGGAGCGGTGTGCCCCAAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606130714_7tpq ATATCATAGACTATAATAGAAAACAACATTTG(+AAAAAA)GATCCATTGGAAGTGCAAAGATAAAGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606130712_p1e8 CATGTAAATACTTTATCTTTGCACTTCCAATG(+AAAAAA)GATCCAAATGTTGTTTTCTATTATAGTCTAT NGG 10 8 17 8 24 0 100 5 10