Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132017_fspf CTATAGAGGGAAACTAGGGTTCAGAAGAGGGG(+AAAAAA)GATCCAAAATCATACAGAGTCCCAGAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606132015_96bc TCACTGCTGCTCTGGGACTCTGTATGATTTTG(+AAAAAA)GATCCCCCTCTTCTGAACCCTAGTTTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606132014_nc6z AGTGAGGGCTCTCAGGTCAGCCACAGAACAGG(+AAAAAA)GATCCACTTAGCTCACTGCCTACTGCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240606132012_zb1q GCCGGGCTCGGCAGTAGGCAGTGAGCTAAGTG(+AAAAAA)GATCCCTGTTCTGTGGCTGACCTGAGAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606132011_g8zp GCCCTCATGATGACAGGGTGCATTTGTAACAG(+AAAAAA)GTACCTTGCACACAGTCAGCCCATCTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606132010_paj0 TCCTACTGTGAGATGGGCTGACTGTGTGCAAG(+AAAAAA)GTACCTGTTACAAATGCACCCTGTCATCATG NGG 10 8 17 8 24 0 100 5 10
    240606132008_ns14 ATCAGGTTAGCACTGGCTGTAGGCTGGTCTGG(+AAAAAA)GATCCTAACAGAATGGCAAGAGGTGAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606132007_mtha ACTGCTTCCCTCACCTCTTGCCATTCTGTTAG(+AAAAAA)GATCCCAGACCAGCCTACAGCCAGTGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240606132005_y9q8 AAAGAATTTAAAGGGGGGGGGGGGGTTTCCAG(+AAAAAA)GTACCCCTGTCCAGGCTTAAACAAAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606132003_6nih AAGGCTCTTGCTTTGTTTAAGCCTGGACAGGG(+AAAAAA)GTACCTGGAAACCCCCCCCCCCCCTTTAAAT NGG 10 8 17 8 24 0 100 5 10