Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130832_4wm3 GGGCAGCACGACCCATACCATGGAAAACAGGG(+AAAAAA)GATCCAGTGTGGGTTCCTCAATGGCCCCTAT NGG 10 8 17 8 24 0 100 5 10
    240606130831_sskn AGAGGATAGGGGCCATTGAGGAACCCACACTG(+AAAAAA)GATCCCCTGTTTTCCATGGTATGGGTCGTGC NGG 10 8 17 8 24 0 100 5 10
    240606130830_xfj6 GCAGTTTTGTGGCAGAATGGGCCATTGTTGTG(+AAAAAA)GATCCATCCAAACGTATCCCCAGCTAAAATA NGG 10 8 17 8 24 0 100 5 10
    240606130829_j63y TCTATTATTTTAGCTGGGGATACGTTTGGATG(+AAAAAA)GATCCACAACAATGGCCCATTCTGCCACAAA NGG 10 8 17 8 24 0 100 5 10
    240606130826_16jd AAAATGTCCTTGATAATAAATGGTATGGCCCG(+AAAAAA)GATCCTATTTTGATAAGATCCAGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606130825_d5tz ACAAATAGCTCCCCTGGATCTTATCAAAATAG(+AAAAAA)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606130823_pus9 ATAGGCCTGTAACTGATCCACTAGGGCAAGGG(+AAAAAA)GTACCTCACAGACAAAAATCACATCCCTAAC NGG 10 8 17 8 24 0 100 5 10
    240606130822_a2i1 CCAGAGTTAGGGATGTGATTTTTGTCTGTGAG(+AAAAAA)GTACCCCTTGCCCTAGTGGATCAGTTACAGG NGG 10 8 17 8 24 0 100 5 10
    240606130819_tqv5 CGGGATTGTTGCCTCTGTCTCCAACACCCTTG(+AAAAAA)GATCCTACCGATCCGTGGGCACACGTCCATA NGG 10 8 17 8 24 0 100 5 10
    240606130818_u9ja AGGATTATGGACGTGTGCCCACGGATCGGTAG(+AAAAAA)GATCCAAGGGTGTTGGAGACAGAGGCAACAA NGG 10 8 17 8 24 0 100 5 10