Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130849_cf6m CTGGGATGGGAGCCACCTTGTTTGGTGATCAG(+AAAAAA)GATCCTGGTGAACTTGTTAGTAAGTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606130847_7i53 GGCCCCTGAGCACTTACTAACAAGTTCACCAG(+AAAAAA)GATCCTGATCACCAAACAAGGTGGCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240606130845_y4uo CTGGGTCTTGGGAGCTCTTGCCTTCACCTGTG(+AAAAAA)GATCCACTTAAGACATTGTGGTCTGAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606130844_88du GGCCTGAGCTTCAGACCACAATGTCTTAAGTG(+AAAAAA)GATCCACAGGTGAAGGCAAGAGCTCCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606130842_f440 GATCTTGCCTGTGAGATCCAGAAGAAGTGTTG(+AAAAAA)GATCCTCTGGAGCTGGAGTTAGAGGTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606130840_kf48 TCTCTCAGCTACCTCTAACTCCAGCTCCAGAG(+AAAAAA)GATCCAACACTTCTTCTGGATCTCACAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606130839_wsm9 CCGACATTCCAAGGAGCTATGGTTGTCACATG(+AAAAAA)GTACCAGCAGCTGGAAGACGGGACTAACGTA NGG 10 8 17 8 24 0 100 5 10
    240606130838_3ty2 AATGATACGTTAGTCCCGTCTTCCAGCTGCTG(+AAAAAA)GTACCATGTGACAACCATAGCTCCTTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240606130837_2g7f AAGTCTGTCATCCTGTGGATGCTATGGGTCTG(+AAAAAA)GATCCTGGGAGCGGGGCCACACTGGCTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606130834_36xf GAAACTCAGAGCCAGTGTGGCCCCGCTCCCAG(+AAAAAA)GATCCAGACCCATAGCATCCACAGGATGACA NGG 10 8 17 8 24 0 100 5 10