Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130817_8m53 TCCCCAGTTGCCAACTCACTGCAGGTGACACG(+AAAAAA)GTACCTCAGGAAGTGTAAGGACCCTTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606130815_h5u1 GGTGGAGGAGAAGGGTCCTTACACTTCCTGAG(+AAAAAA)GTACCGTGTCACCTGCAGTGAGTTGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240606130813_useb GTCAGCTCTTTCCTTTCACATGTGTGATTCAG(+AAAAAA)GTACCTTTACCTGCTGAGCAAGTCCTAAACT NGG 10 8 17 8 24 0 100 5 10
    240606130812_5zc9 TAAGAAGTTTAGGACTTGCTCAGCAGGTAAAG(+AAAAAA)GTACCTGAATCACACATGTGAAAGGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606130810_v5oh TATTCATTTGGAGGTTCTTAATTTGAAGATGG(+AAAAAA)GATCCAGGTTTGGACTGACTGGAGATATTTG NGG 10 8 17 8 24 0 100 5 10
    240606130809_8l3z ATCACCAAATATCTCCAGTCAGTCCAAACCTG(+AAAAAA)GATCCCATCTTCAAATTAAGAACCTCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606130808_25u3 TCCTTATAGCACTCAAGTGGCAGAGGCAGAAG(+AAAAAA)GATCCCTAAGAATTTGAACCAGACTAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240606130806_n15x CTATGTAACCTAGTCTGGTTCAAATTCTTAGG(+AAAAAA)GATCCTTCTGCCTCTGCCACTTGAGTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240606130804_fxnd AAGTCCTCACAGAGGACACAGCTCAGGATCGG(+AAAAAA)GATCCCACACCTGTAAAAAGTAAAGAACAAG NGG 10 8 17 8 24 0 100 5 10
    240606130802_rcav AACCACTTGTTCTTTACTTTTTACAGGTGTGG(+AAAAAA)GATCCCGATCCTGAGCTGTGTCCTCTGTGAG NGG 10 8 17 8 24 0 100 5 10