Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122750_jcxu AGAGACGGTGGTCTCAACTACATGCACAAGTG(+AAAAAA)GATCCTAAAAAATGTATGGAGATTTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606122749_fdtc TTCCATTTAAAAAATCTCCATACATTTTTTAG(+AAAAAA)GATCCACTTGTGCATGTAGTTGAGACCACCG NGG 10 8 17 8 24 0 100 5 10
    240606122747_fntw TGGCTACTTTACGTGTACGTGAATGAGGCTGG(+AAAAAA)GTACCAGATGTAAAGGCTAAGGGAGAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606122745_3wt2 TGTAGTCTCCTCTCCCTTAGCCTTTACATCTG(+AAAAAA)GTACCCAGCCTCATTCACGTACACGTAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606122744_s9ts TTAACTGGAGTTTAATTAATAAACTATTTAGG(+AAAAAA)GATCCACCACCAGTCATATAGACATATTAAA NGG 10 8 17 8 24 0 100 5 10
    240606122742_bidu TTTGTTTTAATATGTCTATATGACTGGTGGTG(+AAAAAA)GATCCCTAAATAGTTTATTAATTAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606122741_co1r CATGTAGTGGCCTGTGGAGGTCATAAACAGTG(+AAAAAA)GATCCCTTGGAGCTGGAGTCACAGTTGGCTA NGG 10 8 17 8 24 0 100 5 10
    240606122738_3s7n TCTCGTAGCCAACTGTGACTCCAGCTCCAAGG(+AAAAAA)GATCCACTGTTTATGACCTCCACAGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240606122737_2d7y CCAAGTTCCTCAGCACTCTCTGAATGACTGTG(+AAAAAA)GATCCTTGAGGAGGAGGAGGAGCAACGCGAA NGG 10 8 17 8 24 0 100 5 10
    240606122736_vkb9 CCGGGTTCGCGTTGCTCCTCCTCCTCCTCAAG(+AAAAAA)GATCCACAGTCATTCAGAGAGTGCTGAGGAA NGG 10 8 17 8 24 0 100 5 10