Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122703_bboq TCCCCTGAGTACTGAGGCTCACCACCCAGCTG(+AAAAAA)GTACCCTCGCCCTGCCTTCTGCCTCTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606122702_xtt3 TAAATCCTTCAGAGGCAGAAGGCAGGGCGAGG(+AAAAAA)GTACCAGCTGGGTGGTGAGCCTCAGTACTCA NGG 10 8 17 8 24 0 100 5 10
    240606122700_vwpk TTCATACATTTAGAAGGCATATAAAGAGATAG(+AAAAAA)GATCCCTTTAGCCCAGGCTGATCTTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240606122659_ea03 ACTACAAGTTCAAGATCAGCCTGGGCTAAAGG(+AAAAAA)GATCCTATCTCTTTATATGCCTTCTAAATGT NGG 10 8 17 8 24 0 100 5 10
    240606122657_paqy AGTGATTAAGAGCCCTGGTTGCTCTTCCAAAG(+AAAAAA)GATCCAGGCTCGATTCCCAGAACTCACATGG NGG 10 8 17 8 24 0 100 5 10
    240606122655_zbte AGCCACCATGTGAGTTCTGGGAATCGAGCCTG(+AAAAAA)GATCCTTTGGAAGAGCAACCAGGGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606122654_ioe9 TGCTCATTAACTGATGTCTCTCCAAAAAGCTG(+AAAAAA)GTACCTCACCTTCAGCTTTGGGCAGAGTCGT NGG 10 8 17 8 24 0 100 5 10
    240606122653_guh2 TCTGCACGACTCTGCCCAAAGCTGAAGGTGAG(+AAAAAA)GTACCAGCTTTTTGGAGAGACATCAGTTAAT NGG 10 8 17 8 24 0 100 5 10
    240606122651_5gsd GCCAGCATGCAAGGCTCTGGGGTCAGTATCTG(+AAAAAA)GTACCAGAGGTGGGGTGGTAGGGTATAAACT NGG 10 8 17 8 24 0 100 5 10
    240606122649_4mle CAGTTAGTTTATACCCTACCACCCCACCTCTG(+AAAAAA)GTACCAGATACTGACCCCAGAGCCTTGCATG NGG 10 8 17 8 24 0 100 5 10