Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122735_aecd ATTCCTGAAAGAAAAGCCAAAATCACAAGCAG(+AAAAAA)GATCCTTTAAAGTCTTTGTGCTCCCAGCGTT NGG 10 8 17 8 24 0 100 5 10
    240606122733_hbo3 GGTAAAACGCTGGGAGCACAAAGACTTTAAAG(+AAAAAA)GATCCTGCTTGTGATTTTGGCTTTTCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606122732_00so TTAAGGCAAGTGTGTCATGGTGCTGAGCGTGG(+AAAAAA)GTACCAATTAGATGTTTCAGTGACACATGCT NGG 10 8 17 8 24 0 100 5 10
    240606122731_3gv9 CACAAAGCATGTGTCACTGAAACATCTAATTG(+AAAAAA)GTACCCACGCTCAGCACCATGACACACTTGC NGG 10 8 17 8 24 0 100 5 10
    240606122729_kpnl CTTGATCCTGATCCACAGTTAATATTGTTATG(+AAAAAA)GATCCTGGTCTACAAAGTGAGTTCCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240606122727_orsi CTGGCTGTCCTGGAACTCACTTTGTAGACCAG(+AAAAAA)GATCCATAACAATATTAACTGTGGATCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606122726_63qz ATGATGTTACTGCTGCATCCAGCTTTTACATG(+AAAAAA)GATCCTAATTGAGGTCCTCATATTTGCATAG NGG 10 8 17 8 24 0 100 5 10
    240606122725_rn5o GCCTCCTATGCAAATATGAGGACCTCAATTAG(+AAAAAA)GATCCATGTAAAAGCTGGATGCAGCAGTAAC NGG 10 8 17 8 24 0 100 5 10
    240606122723_1tm4 GTTCTCGGGGTGGTTCCTCTGTCAGCTGCCAG(+AAAAAA)GATCCTGTGCTAGAGACAGCCTGCCAATGAC NGG 10 8 17 8 24 0 100 5 10
    240606122722_ydhi TCTCTGTCATTGGCAGGCTGTCTCTAGCACAG(+AAAAAA)GATCCTGGCAGCTGACAGAGGAACCACCCCG NGG 10 8 17 8 24 0 100 5 10