Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111313_tl2j AAAAAAACAAACAAAGCAAAAACAAACAAAAG(+AAAAAA)GATCCCCTAGAAACAGTAAGGTAAATCTATT NGG 10 8 17 8 24 0 100 5 10
    240606111311_6o5q AGAAAAATAGATTTACCTTACTGTTTCTAGGG(+AAAAAA)GATCCTTTTGTTTGTTTTTGCTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606111310_gldz TTAGGTAAAATTATAGGAAAAATTATTGAAAG(+AAAAAA)GTACCCTTCAATGGACAGTGTCATCTATTTC NGG 10 8 17 8 24 0 100 5 10
    240606111308_k91s TACTAGAAATAGATGACACTGTCCATTGAAGG(+AAAAAA)GTACCTTTCAATAATTTTTCCTATAATTTTA NGG 10 8 17 8 24 0 100 5 10
    240606111307_vmkh TCTAGAAAGCATATTTAAAATCGTTGGTATCG(+AAAAAA)GTACCAGGAGAAGCCAGGCTAAGAAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240606111305_fc86 CAGGACTTTATTTCTTAGCCTGGCTTCTCCTG(+AAAAAA)GTACCGATACCAACGATTTTAAATATGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606111303_aqkn CACAGCCATGCCCAGCTTTTTGTGAGTGCTGG(+AAAAAA)GATCCAAACTCAGGTACTTCTTACCCACTGA NGG 10 8 17 8 24 0 100 5 10
    240606111302_5dsw ATGTCTCAGTGGGTAAGAAGTACCTGAGTTTG(+AAAAAA)GATCCCAGCACTCACAAAAAGCTGGGCATGG NGG 10 8 17 8 24 0 100 5 10
    240606111301_w977 ACTGTCTTTACACACACTAGAAGAGGGCATTG(+AAAAAA)GATCCCATTACATGTGGTTGCTGGGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240606111259_s43o TGAGTTCAATTCCCAGCAACCACATGTAATGG(+AAAAAA)GATCCAATGCCCTCTTCTAGTGTGTGTAAAG NGG 10 8 17 8 24 0 100 5 10