Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111229_o6eb ATTCAGTAACTTAAATATTGCCAGTGACTGAG(+AAAAAA)GATCCCCTCCAGTCATGGTTCTGTATATTCT NGG 10 8 17 8 24 0 100 5 10
    240606111227_stvt CTCAAAGAATATACAGAACCATGACTGGAGGG(+AAAAAA)GATCCTCAGTCACTGGCAATATTTAAGTTAC NGG 10 8 17 8 24 0 100 5 10
    240606111226_ctem ATAGATACTTTGCATTTATGGCTTGAATGAAG(+AAAAAA)GTACCAATTTTGATCTGGAGTTCTAAATGTG NGG 10 8 17 8 24 0 100 5 10
    240606111224_q4qk ATTTTCACATTTAGAACTCCAGATCAAAATTG(+AAAAAA)GTACCTTCATTCAAGCCATAAATGCAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606111223_2bqv GGCGGCTGTGGTCCAGCAGGATCTACATGTAG(+AAAAAA)GTACCTACTCTTTGGTGCAATAGTGTTACGA NGG 10 8 17 8 24 0 100 5 10
    240606111222_bp8r ATCTTTCGTAACACTATTGCACCAAAGAGTAG(+AAAAAA)GTACCTACATGTAGATCCTGCTGGACCACAG NGG 10 8 17 8 24 0 100 5 10
    240606111220_yxov TAGTTGACCTAGCCTTTCTTGGTGGTTGGTTG(+AAAAAA)GTACCTTTGATGAGATGTTAGAGAATTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606111219_sz20 GAATTACAGAATTCTCTAACATCTCATCAAAG(+AAAAAA)GTACCAACCAACCACCAAGAAAGGCTAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606111218_whpw GTGCCCTGGTGCTGCAGGATTCCAGACTGTAG(+AAAAAA)GATCCACACAGTGAGCTAACACCCCCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606111217_ivmx CTTCATCTGAGGGGGTGTTAGCTCACTGTGTG(+AAAAAA)GATCCTACAGTCTGGAATCCTGCAGCACCAG NGG 10 8 17 8 24 0 100 5 10