Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111201_aq5r CACAAATGACTAAGCACCTGGCAGGGGCTGGG(+AAAAAA)GTACCTTTCAGCAGAGCCTTTGCTGAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606111200_q07l TGCTTCATGCTCAGCAAAGGCTCTGCTGAAAG(+AAAAAA)GTACCCCAGCCCCTGCCAGGTGCTTAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606111158_qj57 CCTGGAGCGCTCTCTCCTGGAGGCAGCGAGGG(+AAAAAA)GTACCACCTCTGCAAATAGACCAGGAAGCGG NGG 10 8 17 8 24 0 100 5 10
    240606111156_rn4p TGGCACCGCTTCCTGGTCTATTTGCAGAGGTG(+AAAAAA)GTACCCCTCGCTGCCTCCAGGAGAGAGCGCT NGG 10 8 17 8 24 0 100 5 10
    240606111155_v78j ATGGACTTCCTTGTCTAAAGGCCACCTCGAGG(+AAAAAA)GTACCACCTCAACATGCCAGGGTACTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606111153_at66 TGAAGAAAACAGTACCCTGGCATGTTGAGGTG(+AAAAAA)GTACCCTCGAGGTGGCCTTTAGACAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606111152_kh9z AGACTGCCAAGTCCGTTCATGTCGCAAGATGG(+AAAAAA)GATCCTGTGGCCCTGAGCCTAATAGTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606111150_7qp6 GGATGGTGGCACTATTAGGCTCAGGGCCACAG(+AAAAAA)GATCCCATCTTGCGACATGAACGGACTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606111147_h51y GGTGTGTTTTTTTTTTTTTCTTTTTCCCCTTG(+AAAAAA)GTACCTATTGGCATTCTGTAAATCACACTAT NGG 10 8 17 8 24 0 100 5 10
    240606111146_0ilv ACCACATAGTGTGATTTACAGAATGCCAATAG(+AAAAAA)GTACCAAGGGGAAAAAGAAAAAAAAAAAAAC NGG 10 8 17 8 24 0 100 5 10