Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111131_gsi0 ACAATAAATGTTTACATTTCTACTAATTTCTG(+AAAAAA)GATCCACTCACCTGTAAATATGCTGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606111129_h60b AACCTGCTGAACAGCATATTTACAGGTGAGTG(+AAAAAA)GATCCAGAAATTAGTAGAAATGTAAACATTT NGG 10 8 17 8 24 0 100 5 10
    240606111128_f94w GTAATCCAGCACCTGGGAGGTAGAGGCTAGTG(+AAAAAA)GATCCTGAGTTCAGGGTCATTCTAGACTATC NGG 10 8 17 8 24 0 100 5 10
    240606111127_1dfu CGATAGATAGTCTAGAATGACCCTGAACTCAG(+AAAAAA)GATCCACTAGCCTCTACCTCCCAGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606111125_3m53 ATCGGCTTGCCTTCTAGATGTATGTGGTCGTG(+AAAAAA)GATCCAGTCTTCAAAAGCCAGATTTTCATAT NGG 10 8 17 8 24 0 100 5 10
    240606111124_cwql GATGTATATGAAAATCTGGCTTTTGAAGACTG(+AAAAAA)GATCCACGACCACATACATCTAGAAGGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606111122_dcsv ATGTATCTTGTTACCTACTCATCCAGCCGTGG(+AAAAAA)GTACCTCGGTTGTTCCCACCCTGGAGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606111120_cc0t TAGTCAGGAGCTCCAGGGTGGGAACAACCGAG(+AAAAAA)GTACCCACGGCTGGATGAGTAGGTAACAAGA NGG 10 8 17 8 24 0 100 5 10
    240606111119_drgf TAAAGGCAGCTGAGCTGCAGGAGCTCTGACAG(+AAAAAA)GATCCGCACGGCATTCCCAGCTGACCGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606111118_3uqc TGCAGTCTCCGGTCAGCTGGGAATGCCGTGCG(+AAAAAA)GATCCTGTCAGAGCTCCTGCAGCTCAGCTGC NGG 10 8 17 8 24 0 100 5 10