Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111215_8jn5 CAACTTAGAAGAGCAGGCAGTTTAGCCTGGGG(+AAAAAA)GATCCAGACACAGTGGGGCCTGGAAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240606111213_w3j9 GCAGACCCCATTTCCAGGCCCCACTGTGTCTG(+AAAAAA)GATCCCCCAGGCTAAACTGCCTGCTCTTCTA NGG 10 8 17 8 24 0 100 5 10
    240606111212_ccay GGCAGGGCTCATATCTATTGGCAAAATTCAAG(+AAAAAA)GTACCTGTAAGACACTTGCCTGGGCTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606111211_d39j ACGGATCCAGAGCCCAGGCAAGTGTCTTACAG(+AAAAAA)GTACCTTGAATTTTGCCAATAGATATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606111210_a79m AAGGTACCTGTAAGACACTTGCCTGGGCTCTG(+AAAAAA)GATCCGTTTCCTGGGTGCCTAGGACCTCACG NGG 10 8 17 8 24 0 100 5 10
    240606111209_d1wz CAGACCGTGAGGTCCTAGGCACCCAGGAAACG(+AAAAAA)GATCCAGAGCCCAGGCAAGTGTCTTACAGGT NGG 10 8 17 8 24 0 100 5 10
    240606111207_q539 CCCCAGCAATCCAGTCATCTCCTGTCAGGTTG(+AAAAAA)GATCCAGGAGAAGTGAGGTCAAAACTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606111205_tglq TTGACTCTTCAGTTTTGACCTCACTTCTCCTG(+AAAAAA)GATCCAACCTGACAGGAGATGACTGGATTGC NGG 10 8 17 8 24 0 100 5 10
    240606111204_2jzk TCCTCATCATGGCTTCCCAGACCACCTCACTG(+AAAAAA)GTACCTTTTGCTATGTTAGCTTGTTATGAGT NGG 10 8 17 8 24 0 100 5 10
    240606111203_mf5g AGCTAACTCATAACAAGCTAACATAGCAAAAG(+AAAAAA)GTACCAGTGAGGTGGTCTGGGAAGCCATGAT NGG 10 8 17 8 24 0 100 5 10