Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111258_lya9 TGACCCGTGCTTTGAGAACTCGGGAAAACCAG(+AAAAAA)GATCCATGTCCACTTCACTTCGACTTTATCT NGG 10 8 17 8 24 0 100 5 10
    240606111256_e0dj TGAGGAGATAAAGTCGAAGTGAAGTGGACATG(+AAAAAA)GATCCTGGTTTTCCCGAGTTCTCAAAGCACG NGG 10 8 17 8 24 0 100 5 10
    240606111255_ddu5 CATGTAACGGAAGGGTGGGCGCAGATCCTTAG(+AAAAAA)GTACCAGGGTGCTGTGCAATGGAAGATGCAG NGG 10 8 17 8 24 0 100 5 10
    240606111252_n59e CATCTCTGCATCTTCCATTGCACAGCACCCTG(+AAAAAA)GTACCTAAGGATCTGCGCCCACCCTTCCGTT NGG 10 8 17 8 24 0 100 5 10
    240606111250_v7an ACACACACACACACAAATACTCAAGAGGTGGG(+AAAAAA)GATCCACAAATATGAAGTAAACCAGAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606111249_vzqz AATCAACTGCTCTGGTTTACTTCATATTTGTG(+AAAAAA)GATCCCCACCTCTTGAGTATTTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606111248_xe40 AGTAAGAGTTCCATTTTCTCACATCAGTGAAG(+AAAAAA)GTACCATACATTGAAACTCTCAGTACTATAT NGG 10 8 17 8 24 0 100 5 10
    240606111247_dq10 TAGAAATATAGTACTGAGAGTTTCAATGTATG(+AAAAAA)GTACCTTCACTGATGTGAGAAAATGGAACTC NGG 10 8 17 8 24 0 100 5 10
    240606111246_g2tn AGAATTCAATATAGTTGTTGCTGTGAATATAG(+AAAAAA)GTACCATTTTGGGTCATCTACACTAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240606111245_b4u7 TGTGTCAGTCTTAGTGTAGATGACCCAAAATG(+AAAAAA)GTACCTATATTCACAGCAACAACTATATTGA NGG 10 8 17 8 24 0 100 5 10