Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224754_2x29 ATCCTTTTGAGAGAAGAAAGAACACTAACATG(+CAGTGT)GTACCAACCGAGACAGGGGGAGAAAACACAC NGG 10 8 17 8 24 0 100 5 10
    240605224752_vx14 GCTAGCTAAGGCTCTGTAGCCCTCATCTCACG(+CAGTGT)GATCCTCCAGCTCTACAGCAATGTCGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605224751_z52r CCCTGCAGCCCGACATTGCTGTAGAGCTGGAG(+CAGTGT)GATCCGTGAGATGAGGGCTACAGAGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240605224749_fl9q TGCAGACCTCCGCAGCCAGTCCTCAGCGATTG(+CAGTGT)GTACCTACCTGGTCCCGGATAAATAAACGAT NGG 10 8 17 8 24 0 100 5 10
    240605224748_gdrz ACAAAATCGTTTATTTATCCGGGACCAGGTAG(+CAGTGT)GTACCAATCGCTGAGGACTGGCTGCGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605224745_rxbk AGCAGTTTAGAAGCCATGAGTCTGGGTGCCTG(+CAGTGT)GATCCTCACCGAGGCCCGGGGAGTATCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605224744_epz3 GGAGAGCAAGATACTCCCCGGGCCTCGGTGAG(+CAGTGT)GATCCAGGCACCCAGACTCATGGCTTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605224742_56k3 AAATAAAATAAATAGAATAAAGTATTCAAAGG(+CAGTGT)GTACCACCACTCCAATTGCATCAAGCCACCT NGG 10 8 17 8 24 0 100 5 10
    240605224741_6hh7 GTCCAAGGTGGCTTGATGCAATTGGAGTGGTG(+CAGTGT)GTACCCTTTGAATACTTTATTCTATTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605224740_jaaw AAGTATTGTTTCCTAATCAGACTTTTACATTG(+CAGTGT)GTACCATGAGTTCTTTATGCTTTGCCAAGAC NGG 10 8 17 8 24 0 100 5 10