Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224738_oe8a ACAATGTCTTGGCAAAGCATAAAGAACTCATG(+CAGTGT)GTACCAATGTAAAAGTCTGATTAGGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605224736_dqs5 ACAACCTTGCGAGCAACTTGAGCTTCCTCCAG(+CAGTGT)GATCCTGCTTGATTAGACCCTCAGAGATAGC NGG 10 8 17 8 24 0 100 5 10
    240605224735_r5kt CAATGGCTATCTCTGAGGGTCTAATCAAGCAG(+CAGTGT)GATCCTGGAGGAAGCTCAAGTTGCTCGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605224733_hlnh TATCCCGAGGACAGTTACTAAGCTATACTTGG(+CAGTGT)GATCCCACTCAGAAGGTTTCAGAACTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605224732_8vrf GAAGGACCCCAGTTCTGAAACCTTCTGAGTGG(+CAGTGT)GATCCCAAGTATAGCTTAGTAACTGTCCTCG NGG 10 8 17 8 24 0 100 5 10
    240605224729_iirl CCATCCACACCCTGCAGCAAGTCCCAACCTGG(+CAGTGT)GTACCACTGTTATTATTACAAGGAAAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605224728_lyl4 TTTAACTTTCTTTCCTTGTAATAATAACAGTG(+CAGTGT)GTACCCAGGTTGGGACTTGCTGCAGGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605224727_hryy AGAGAGGGATGTGGTGTGGACTCTGAGCTGAG(+CAGTGT)GATCCTCTCCACGTTGTGATGGGGTAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605224725_a0rj CGGCCCCTCCTACCCCATCACAACGTGGAGAG(+CAGTGT)GATCCTCAGCTCAGAGTCCACACCACATCCC NGG 10 8 17 8 24 0 100 5 10
    240605224723_va0e GGGAATCTGACACCTTCCGCTGGGTTCTGAGG(+CAGTGT)GTACCTGTACTCATGTGTACCCTGCCCCCTC NGG 10 8 17 8 24 0 100 5 10