Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224704_8fwd GGGGTGGGGCTGCACGGGGAGCCCTTCTGAGG(+CAGTGT)GATCCAATCTCCTCCTTCTCCGCTGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605224702_4ge7 AGATTTTGAGATCACATCGTTCTCCCCTCAAG(+CAGTGT)GATCCCCTGATTAGTGGGAGACTAAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240605224700_m3hf GGGGGCAGGTCTTAGTCTCCCACTAATCAGGG(+CAGTGT)GATCCTTGAGGGGAGAACGATGTGATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605224658_5tc9 TGAGAGAAGGGCTGGGAGCCAGCCAAGGGATG(+CAGTGT)GTACCTTTGTTGTGACGGGGCTACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605224657_y32d GCTGAGAAGGGTGTAGCCCCGTCACAACAAAG(+CAGTGT)GTACCATCCCTTGGCTGGCTCCCAGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605224656_t1tj AGAAGTCCAGATACTCCATTCTTGAGTGTCAG(+CAGTGT)GATCCCAGGCAAAACTGGCAATACAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605224653_783p GTTTCCTGGATTGTATTGCCAGTTTTGCCTGG(+CAGTGT)GATCCTGACACTCAAGAATGGAGTATCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605224652_p2vw GTGCTTGCTGCCAGGTTTGACCACGTGAGTTG(+CAGTGT)GATCCCTGGAACCCACATGGTGGAAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605224650_amgo CAATTCTCTCTTTCCACCATGTGGGTTCCAGG(+CAGTGT)GATCCAACTCACGTGGTCAAACCTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605224649_xzb6 GTGGTCATTGAGTGCCACCAGTACATCTGATG(+CAGTGT)GATCCAGTTGGCACGTCATGACAGATTGACT NGG 10 8 17 8 24 0 100 5 10