Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224559_jq2f CTCCCGTAGCCATGGACTCGGGAAAAGAGTAG(+CAGTGT)GTACCATCGTGTTTTGTGTTTGATGCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605224557_hxr0 ATTTGGATTTAAATGGATTTGAAAGTCTTTGG(+CAGTGT)GATCCTTGAAGGATTTGTAATAATCACTTTC NGG 10 8 17 8 24 0 100 5 10
    240605224556_gdpk TTAGAGAAAGTGATTATTACAAATCCTTCAAG(+CAGTGT)GATCCCAAAGACTTTCAAATCCATTTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605224554_ewna GGCCCCGGTAATTAAGAGGTGAAATTCGCCTG(+CAGTGT)GTACCAAGTGGGGCTGTGAACTTAAATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605224553_5uet AATTATTTAATTTAAGTTCACAGCCCCACTTG(+CAGTGT)GTACCAGGCGAATTTCACCTCTTAATTACCG NGG 10 8 17 8 24 0 100 5 10
    240605224550_5pjd TTTCAAGTATCGTGATGGATGAAGATGGGGGG(+CAGTGT)GATCCTTCGCTTACATCTCCACACTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605224549_dmbi CTGAAAACCAAAGTGTGGAGATGTAAGCGAAG(+CAGTGT)GATCCCCCCCATCTTCATCCATCACGATACT NGG 10 8 17 8 24 0 100 5 10
    240605224547_htxc TTGAGCTCAAGTGAATTGACTTTTTTTTGACG(+CAGTGT)GATCCAGAAGACCACATGAGATTTCCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605224546_kwwz TTTACCTCCTGGAAATCTCATGTGGTCTTCTG(+CAGTGT)GATCCGTCAAAAAAAAGTCAATTCACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605224544_qg4r GACACTCATATCCATTGGCACTGACAGCCTTG(+CAGTGT)GTACCAGAAGGTTAGGTGAATGAACGGGGTC NGG 10 8 17 8 24 0 100 5 10