Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224511_3m8t TTTTGTTAGTGTCACGCTTCCTGGTTGACAAG(+CAGTGT)GATCCTCAGCCTCATGACATACATTCTTACC NGG 10 8 17 8 24 0 100 5 10
    240605224509_qy6d GTTAAGTGAACCTCTTCTAGGTGGCCCACATG(+CAGTGT)GTACCTTGGGGACAAGTGAAGTTATGAATGA NGG 10 8 17 8 24 0 100 5 10
    240605224507_e0p4 AATCCTCATTCATAACTTCACTTGTCCCCAAG(+CAGTGT)GTACCATGTGGGCCACCTAGAAGAGGTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605224505_whx8 GTTAAGTGAACCTCTTCTAGGTGGCCCACATG(+CAGTGT)GTACCTTGGGGACAAGTGAAGTTATGAATGA NGG 10 8 17 8 24 0 100 5 10
    240605224502_v5sk AATCCTCATTCATAACTTCACTTGTCCCCAAG(+CAGTGT)GTACCATGTGGGCCACCTAGAAGAGGTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605224501_r5qn AGTACTTGTAAACCTAGGTGATTCTATCTTTG(+CAGTGT)GTACCATTGTGAGCAGTACTCCCCAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605224459_vqox GCAGCAGAGGCTGGGGAGTACTGCTCACAATG(+CAGTGT)GTACCAAAGATAGAATCACCTAGGTTTACAA NGG 10 8 17 8 24 0 100 5 10
    240605224458_4r2n GCCTGCCGTGTGAGGCCCAACGCATAGAGCAG(+CAGTGT)GTACCTGACTGCGTCCAGCCTTTCTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605224457_b23f CCAAGCTGCCCAGAAAGGCTGGACGCAGTCAG(+CAGTGT)GTACCTGCTCTATGCGTTGGGCCTCACACGG NGG 10 8 17 8 24 0 100 5 10
    240605224455_k9s5 CCGCTGGATGCATGGCCTGTGGGACCTCTGTG(+CAGTGT)GTACCAGAGGTCCATTCCGTTCCTTCGCAGC NGG 10 8 17 8 24 0 100 5 10