Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224358_osaf CAGCATCCATTGTCGTTAGTTTGTACTTTGGG(+CAGTGT)GTACCCATTAATCACAGCAGGGACCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605224356_8x08 GAGCGCAGTGGGATGAACTCACAGCAGTACTG(+CAGTGT)GTACCATTTTAGATTTCTTTCTTGCACTATA NGG 10 8 17 8 24 0 100 5 10
    240605224355_75nu TGAAGTATAGTGCAAGAAAGAAATCTAAAATG(+CAGTGT)GTACCAGTACTGCTGTGAGTTCATCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605224354_w7dv TCTGGGGATGTGGGATCTGCTTCTAAGTGTGG(+CAGTGT)GTACCATGTCTACTCTCACTTTATAAAATTA NGG 10 8 17 8 24 0 100 5 10
    240605224352_l62z AAGGTTAATTTTATAAAGTGAGAGTAGACATG(+CAGTGT)GTACCCACACTTAGAAGCAGATCCCACATCC NGG 10 8 17 8 24 0 100 5 10
    240605224349_oz46 CCTGTAGCAGGCTCTGCTTTTCGGGTCCCATG(+CAGTGT)GTACCAGATTCTTGAAATAAGGTCATGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605224348_t5jm CTAACTCTTCATGACCTTATTTCAAGAATCTG(+CAGTGT)GTACCATGGGACCCGAAAAGCAGAGCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605224346_41xj GCTGATGATATGTACGTGCCTGAAACTGGATG(+CAGTGT)GTACCTTTAACTGACACTTAGAAAGCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605224345_hldh GCTAATAAAAGCTTTCTAAGTGTCAGTTAAAG(+CAGTGT)GTACCATCCAGTTTCAGGCACGTACATATCA NGG 10 8 17 8 24 0 100 5 10
    240605224344_fyyr CAGTGCACTGCACCTCGGAACACATGCAGTTG(+CAGTGT)GATCCTAAGGATGTTTAAAAGCGATTTTAAA NGG 10 8 17 8 24 0 100 5 10