Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224341_lket GAGACTTTAAAATCGCTTTTAAACATCCTTAG(+CAGTGT)GATCCAACTGCATGTGTTCCGAGGTGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605224340_2a89 ACAGCCGCATTCCAGGGTGGGAGAGCGTGCTG(+CAGTGT)GTACCATCTTCTCTCAAGTAGCTCTGTCATC NGG 10 8 17 8 24 0 100 5 10
    240605224339_g19c GCCCCGATGACAGAGCTACTTGAGAGAAGATG(+CAGTGT)GTACCAGCACGCTCTCCCACCCTGGAATGCG NGG 10 8 17 8 24 0 100 5 10
    240605224338_rftk CTCACAAAATCTGATGACATTTTGTGAAGTTG(+CAGTGT)GTACCATCTCTACATGCGCCTAAGTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240605224336_shbo ATGCCCCTGTCACTTAGGCGCATGTAGAGATG(+CAGTGT)GTACCAACTTCACAAAATGTCATCAGATTTT NGG 10 8 17 8 24 0 100 5 10
    240605224334_m5rb CATTCATGTAGCATATGCCTCCATTTTTCCTG(+CAGTGT)GTACCTAGCATCAGAAGTAACATTTATGAAC NGG 10 8 17 8 24 0 100 5 10
    240605224333_b5l6 AGAGAGTTCATAAATGTTACTTCTGATGCTAG(+CAGTGT)GTACCAGGAAAAATGGAGGCATATGCTACAT NGG 10 8 17 8 24 0 100 5 10
    240605224332_oeqv TGGGCTCCCAAGCTTGTCTTTGGGTAGAGATG(+CAGTGT)GATCCAGTCAGTGTTAGGACACTGGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605224330_hpm2 TATCTGCTTCCCCAGTGTCCTAACACTGACTG(+CAGTGT)GATCCATCTCTACCCAAAGACAAGCTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605224329_fv8s TTTGGCTGTAGCTTCAGTTCCACTCTGAACTG(+CAGTGT)GATCCTCTTGGTAGCTGGACTTAATAAAATG NGG 10 8 17 8 24 0 100 5 10