Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224327_bcvw CACCTCATTTTATTAAGTCCAGCTACCAAGAG(+CAGTGT)GATCCAGTTCAGAGTGGAACTGAAGCTACAG NGG 10 8 17 8 24 0 100 5 10
    240605224326_4gar CCACTGTGAGCATAAGCCTGGACTCTGCAAAG(+CAGTGT)GATCCCACAGGTAGCCACATCTTTTAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605224325_buw8 GTCTATCTTTTAAAAGATGTGGCTACCTGTGG(+CAGTGT)GATCCTTTGCAGAGTCCAGGCTTATGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605224323_a5go AGTGGTGAAGTGTTCTTACCACCCCAGCTCAG(+CAGTGT)GATCCTTGAAGCCTGGGGTTGTGAGAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605224321_v45a AATTGAGTTTTCTCACAACCCCAGGCTTCAAG(+CAGTGT)GATCCTGAGCTGGGGTGGTAAGAACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240605224319_ywkb TGGCTCATCTCCTTTTGTCTGGACATCCTGGG(+CAGTGT)GTACCAAGAAATACACCTGAGAAGAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240605224318_v5ww TGTGTGTTTGTTCTTCTCAGGTGTATTTCTTG(+CAGTGT)GTACCCCAGGATGTCCAGACAAAAGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605224316_4vfg GTCCTTGTAATGAGAATTCCCTCTCTTTGAAG(+CAGTGT)GTACCCAGCCTAGCCTCCAGGCCTCAACATT NGG 10 8 17 8 24 0 100 5 10
    240605224315_lg9j CGCCAAATGTTGAGGCCTGGAGGCTAGGCTGG(+CAGTGT)GTACCTTCAAAGAGAGGGAATTCTCATTACA NGG 10 8 17 8 24 0 100 5 10
    240605224313_xryg TCTGATTCAAGAAATAGCCTGGGTCAGAAGAG(+CAGTGT)GTACCCCAGGTAAAATTGAGAGAGCATTAGG NGG 10 8 17 8 24 0 100 5 10