Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224235_lrzi TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+CAGTGT)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605224233_37uk GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+CAGTGT)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605224232_z9e9 TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+CAGTGT)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605224230_50tp GCCTCCACCTCCAAGTGCTGGATTTACGGAGG(+CAGTGT)GATCCAACCTAAGGCCTCTAACAAACACACA NGG 10 8 17 8 24 0 100 5 10
    240605224228_w55l GTGCTTGTGTGTTTGTTAGAGGCCTTAGGTTG(+CAGTGT)GATCCCTCCGTAAATCCAGCACTTGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605224227_ho21 GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+CAGTGT)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605224226_w57i TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+CAGTGT)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605224224_9qtj CAGCAAACTTGACCCCTGATTTTAACCCTCAG(+CAGTGT)GATCCACACGGAAGGGGAAAACTCACTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605224223_r9vg ACTTTCAGGAGTGAGTTTTCCCCTTCCGTGTG(+CAGTGT)GATCCTGAGGGTTAAAATCAGGGGTCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605224221_nj2j CAGGGATCTTCAAGGACAGGCCTGGGTTTGGG(+CAGTGT)GATCCCACCTCTTGAGTATGAAAGAATTCAG NGG 10 8 17 8 24 0 100 5 10