Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224219_y5aj CACTCCTGAATTCTTTCATACTCAAGAGGTGG(+CAGTGT)GATCCCCAAACCCAGGCCTGTCCTTGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605224216_m6w7 GCTGCCTACTGTCTATAACTCCAGATCCAGGG(+CAGTGT)GATCCTATACTTTTCTCTAGCCTATTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240605224215_cvx3 CAGGTGTCCAAATAGGCTAGAGAAAAGTATAG(+CAGTGT)GATCCCCTGGATCTGGAGTTATAGACAGTAG NGG 10 8 17 8 24 0 100 5 10
    240605224213_fxyi GCCTGTCATGATGGTGCTGGGAAACAAACTTG(+CAGTGT)GATCCTCTGAAAGAGCAGTGAGTACTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605224212_x0cj AGTGGTTAAAAGTACTCACTGCTCTTTCAGAG(+CAGTGT)GATCCAAGTTTGTTTCCCAGCACCATCATGA NGG 10 8 17 8 24 0 100 5 10
    240605224211_a8dr CTGGTGCTCAAGAAAGCTAGCAGAGGGCGCTG(+CAGTGT)GATCCCATGGAACTGGAGTTAGGGAGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605224208_kc97 GCTTATCACCCTCCCTAACTCCAGTTCCATGG(+CAGTGT)GATCCAGCGCCCTCTGCTAGCTTTCTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605224207_jlx2 CCTCTAAGTGCCAAACAGACCATGCCCGCTGG(+CAGTGT)GATCCGGATCAGAAGAAAGCAGGATCGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605224205_t9v9 GGGCTCACTCGATCCTGCTTTCTTCTGATCCG(+CAGTGT)GATCCCAGCGGGCATGGTCTGTTTGGCACTT NGG 10 8 17 8 24 0 100 5 10
    240605224203_5dyu ATAACACTGCCAATGGTTAAGTCACAGTCCTG(+CAGTGT)GATCCTTAGCTATTACCATCCCTCAGAAAGC NGG 10 8 17 8 24 0 100 5 10