Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224117_ivbi CAATAAAGGAACAAAAGAACCCAGCAATCCAG(+CAGTGT)GTACCATGATCTTGCTCAAGTAGAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605224115_blh6 GAGGGCATCTCTTCTACTTGAGCAAGATCATG(+CAGTGT)GTACCTGGATTGCTGGGTTCTTTTGTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605224114_o63n TTTAAGTTTCATAACTGTACTAGAGATGGCAG(+CAGTGT)GATCCTGCCCAGCACAGTCTTTCCGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605224112_04vw ATGTCATTAAACGGAAAGACTGTGCTGGGCAG(+CAGTGT)GATCCTGCCATCTCTAGTACAGTTATGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605224110_etzg AGGAGACTAATGCCCTCTGATGATCTCTATGG(+CAGTGT)GTACCAGGCACACATAGACATACAGGTATGC NGG 10 8 17 8 24 0 100 5 10
    240605224109_kqol TACCTGCATACCTGTATGTCTATGTGTGCCTG(+CAGTGT)GTACCCATAGAGATCATCAGAGGGCATTAGT NGG 10 8 17 8 24 0 100 5 10
    240605224107_rhs0 AGTGGTTCAGAGAACTGGTTGTTCTTGCAGAG(+CAGTGT)GATCCTAGGTTCAGTTCCTAGTACCTGCATG NGG 10 8 17 8 24 0 100 5 10
    240605224105_9h7c TCTGCCATGCAGGTACTAGGAACTGAACCTAG(+CAGTGT)GATCCTCTGCAAGAACAACCAGTTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605224103_931o ACCCTCATACTGTCAGATACGTTTTGATAAGG(+CAGTGT)GATCCAAGAGCAGTAAGGGTCAGTCTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605224102_6k64 CTTTGTTGAAAGACTGACCCTTACTGCTCTTG(+CAGTGT)GATCCCTTATCAAAACGTATCTGACAGTATG NGG 10 8 17 8 24 0 100 5 10