Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224044_fzmc GAACACGAGGTCAGAAGCTTCTCTTCTATGTG(+CAGTGT)GATCCTTGAACTAGTCCGGCCGGCGTGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605224043_md97 GTTCGACTTCACGCCGGCCGGACTAGTTCAAG(+CAGTGT)GATCCACATAGAAGAGAAGCTTCTGACCTCG NGG 10 8 17 8 24 0 100 5 10
    240605224041_36br AACATTTCTGACTACATTATAAAGCACCACAG(+CAGTGT)GTACCAGGCGATTTCTGTATGCTTATTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605224039_l3um TCAGTTCAAAATAAGCATACAGAAATCGCCTG(+CAGTGT)GTACCTGTGGTGCTTTATAATGTAGTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605224037_cl7b GCCTCCAACTCCCATTGACAGCCTCAATGAGG(+CAGTGT)GTACCATACATCATGAGATAAGTATGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605224036_if2e ACATGTTCTTCATACTTATCTCATGATGTATG(+CAGTGT)GTACCCTCATTGAGGCTGTCAATGGGAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605224034_zqsc TTTTGTATTTATTTGTATGTTCAGATCCAAAG(+CAGTGT)GATCCGTTGTAAAAATATATATGTGAATATA NGG 10 8 17 8 24 0 100 5 10
    240605224033_iy3m GTATATATATTCACATATATATTTTTACAACG(+CAGTGT)GATCCTTTGGATCTGAACATACAAATAAATA NGG 10 8 17 8 24 0 100 5 10
    240605224031_eqy4 TGGCATACTTATGCCATATCTGTTTTGATTGG(+CAGTGT)GATCCTGGAACCTCCCTTAAATCAAGGATTG NGG 10 8 17 8 24 0 100 5 10
    240605224029_xig3 AAGGCCAATCCTTGATTTAAGGGAGGTTCCAG(+CAGTGT)GATCCCAATCAAAACAGATATGGCATAAGTA NGG 10 8 17 8 24 0 100 5 10