Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223944_2i89 TTTATAAGGAAAAGACTCAAGGTATAAGCTCG(+CAGTGT)GATCCCTAACCCTGTGCTATGTCATCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605223943_ja1f GTGAGAGAAAGATGACATAGCACAGGGTTAGG(+CAGTGT)GATCCGAGCTTATACCTTGAGTCTTTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605223941_as66 TCAGTCCAGAACTAAAAATCAATGTGAAAATG(+CAGTGT)GATCCTCACCTTGAAAGACAAGTGTGACTTC NGG 10 8 17 8 24 0 100 5 10
    240605223940_69hy AGAAAGAAGTCACACTTGTCTTTCAAGGTGAG(+CAGTGT)GATCCATTTTCACATTGATTTTTAGTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605223939_t1et AGAAAAAATGTGAATACACTTGCTGAGAATTG(+CAGTGT)GATCCTATATATAAAATATCATACCCACATT NGG 10 8 17 8 24 0 100 5 10
    240605223938_tmkg TGTTTAATGTGGGTATGATATTTTATATATAG(+CAGTGT)GATCCAATTCTCAGCAAGTGTATTCACATTT NGG 10 8 17 8 24 0 100 5 10
    240605223936_pq2v AAGCAGCTTTGTTGCTGCCTTTATGGACATGG(+CAGTGT)GTACCAATTGCTTTTTGTAATATGGTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605223934_135e TCACATTTTTACCATATTACAAAAAGCAATTG(+CAGTGT)GTACCCATGTCCATAAAGGCAGCAACAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605223933_e5uf GGGTAGAATCCTTCCAGAAGCCATGCTTTCAG(+CAGTGT)GATCCACTGTGATTGTCCAGCAGGGCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605223932_o79u TCATGAGCTAGCCCTGCTGGACAATCACAGTG(+CAGTGT)GATCCTGAAAGCATGGCTTCTGGAAGGATTC NGG 10 8 17 8 24 0 100 5 10