Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224014_4ejh TCAGTGGACCCAGGGTTGGGAGGAGCTCAAGG(+CAGTGT)GATCCTCCTGCCTCTGTGTCCCAAGCGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605224013_jgnt AGTCCTTAGCGCTTGGGACACAGAGGCAGGAG(+CAGTGT)GATCCCTTGAGCTCCTCCCAACCCTGGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605224011_ks37 TATAACCAGACAGAAAGTGAAGCTGGTTAGTG(+CAGTGT)GATCCAATGTAACTCCCGGAACTGGGGTAAT NGG 10 8 17 8 24 0 100 5 10
    240605224010_4mhz ACCTCATTACCCCAGTTCCGGGAGTTACATTG(+CAGTGT)GATCCACTAACCAGCTTCACTTTCTGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605224008_pgjm TTTATGTGTATTGTATACCACATGTGTGCCTG(+CAGTGT)GTACCTGTGAAGGTCAGAAGAGGCCATCAGC NGG 10 8 17 8 24 0 100 5 10
    240605224006_dboj GTAGAGCTGATGGCCTCTTCTGACCTTCACAG(+CAGTGT)GTACCAGGCACACATGTGGTATACAATACAC NGG 10 8 17 8 24 0 100 5 10
    240605224005_585l TGTATTCGTTGTATGTGTGTACACACAGTCTG(+CAGTGT)GTACCTGACATTCAACAGTGACTCTTTATAG NGG 10 8 17 8 24 0 100 5 10
    240605224004_wt05 CCAGGCTATAAAGAGTCACTGTTGAATGTCAG(+CAGTGT)GTACCAGACTGTGTGTACACACATACAACGA NGG 10 8 17 8 24 0 100 5 10
    240605224003_mqhq ATAGTGCCCCTGTGGCCCAGAAGAGAGTGTTG(+CAGTGT)GATCCCTGAGACTGGAGTTATAGACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605224001_hqdj AGTTCACTTCTGTCTATAACTCCAGTCTCAGG(+CAGTGT)GATCCAACACTCTCTTCTGGGCCACAGGGGC NGG 10 8 17 8 24 0 100 5 10