Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223913_5ncc GGGGACCCAATTCTTTCTTCTGGCCTCCAAGG(+CAGTGT)GTACCAGCCATACATACATACATACATACAT NGG 10 8 17 8 24 0 100 5 10
    240605223912_ikk8 TATGTATGTATGTATGTATGTATGTATGGCTG(+CAGTGT)GTACCCTTGGAGGCCAGAAGAAAGAATTGGG NGG 10 8 17 8 24 0 100 5 10
    240605223910_icmb CCCAACCCCTCTAAACCAGCTCTGTGGCTCAG(+CAGTGT)GTACCTTCAGTGAAGCCCATTGCTCTTGCCG NGG 10 8 17 8 24 0 100 5 10
    240605223909_b71u CTGGGCGGCAAGAGCAATGGGCTTCACTGAAG(+CAGTGT)GTACCTGAGCCACAGAGCTGGTTTAGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605223908_0mr5 GAATGAGATTTTGTATTTTCCCTTGAGAGATG(+CAGTGT)GTACCAAGCAGGCAAGCAACCATGTACCTAC NGG 10 8 17 8 24 0 100 5 10
    240605223906_9ya5 AACTGGTAGGTACATGGTTGCTTGCCTGCTTG(+CAGTGT)GTACCATCTCTCAAGGGAAAATACAAAATCT NGG 10 8 17 8 24 0 100 5 10
    240605223905_xwp9 AATTGGGTTTTGTCTTCTTTAAAAACAAATAG(+CAGTGT)GTACCGTAAGCATTTTCTGCTGACGACTGCC NGG 10 8 17 8 24 0 100 5 10
    240605223903_865r CTTAAGGCAGTCGTCAGCAGAAAATGCTTACG(+CAGTGT)GTACCTATTTGTTTTTAAAGAAGACAAAACC NGG 10 8 17 8 24 0 100 5 10
    240605223902_8ki7 TGAATAAGAACCAAACTGAGTATTATAGTGAG(+CAGTGT)GATCCTTGACCACTTAGTAGACCTCAGCAAA NGG 10 8 17 8 24 0 100 5 10
    240605223900_npy3 AGAATTTTGCTGAGGTCTACTAAGTGGTCAAG(+CAGTGT)GATCCTCACTATAATACTCAGTTTGGTTCTT NGG 10 8 17 8 24 0 100 5 10