Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223825_xhh1 CAGGAGTCAGCTCTTTTTCTTGGGAGTAAGAG(+CAGTGT)GTACCAAAATAAGCAGAAAACAAAAAAATCA NGG 10 8 17 8 24 0 100 5 10
    240605223824_85eq TTAGTTGATTTTTTTGTTTTCTGCTTATTTTG(+CAGTGT)GTACCTCTTACTCCCAAGAAAAAGAGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240605223822_wxyn ACCTCTGTGATGGGCTGCTTGTCCGGGACCAG(+CAGTGT)GTACCATGCTTAGGCTAAGTGCATGATGTCA NGG 10 8 17 8 24 0 100 5 10
    240605223820_xax1 CACAGTGACATCATGCACTTAGCCTAAGCATG(+CAGTGT)GTACCTGGTCCCGGACAAGCAGCCCATCACA NGG 10 8 17 8 24 0 100 5 10
    240605223819_4oxr ACAGCATTCCGAGTACATGGACACTACATACG(+CAGTGT)GTACCAGCTCCCTGGAAAAGATAAATATTGG NGG 10 8 17 8 24 0 100 5 10
    240605223817_nuwo TCGATCCAATATTTATCTTTTCCAGGGAGCTG(+CAGTGT)GTACCGTATGTAGTGTCCATGTACTCGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605223816_msni AGTAAGGAGGGGACTGAGTACGTAACCTGTTG(+CAGTGT)GTACCCTTAAACTGGACAAACGTCACAATGC NGG 10 8 17 8 24 0 100 5 10
    240605223814_ekkl ATGTGGCATTGTGACGTTTGTCCAGTTTAAGG(+CAGTGT)GTACCAACAGGTTACGTACTCAGTCCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605223812_55cp TACTGACCAGTTCAGATGGGTCCACTAGAGGG(+CAGTGT)GATCCTTGAGCATGCACACTTGGAAAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605223811_jyyf CATGGGTGTTTTTCCAAGTGTGCATGCTCAAG(+CAGTGT)GATCCCCTCTAGTGGACCCATCTGAACTGGT NGG 10 8 17 8 24 0 100 5 10