Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223840_m6tf AAAAGAATATACCTTCTTCTCAGCACCTCATG(+CAGTGT)GTACCTTCTCCAAAATTGACCATATAATCGG NGG 10 8 17 8 24 0 100 5 10
    240605223839_yh9s TGTGACCGATTATATGGTCAATTTTGGAGAAG(+CAGTGT)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605223837_fps4 CCTCTTCCAAACGACCCTAGTTCAATTTCCAG(+CAGTGT)GATCCACATGGCAACTCATAGATGTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605223836_3l82 TGAAGTTACAGACATCTATGAGTTGCCATGTG(+CAGTGT)GATCCTGGAAATTGAACTAGGGTCGTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605223835_4lvt TCAGGGCAGAGAAACTGAGGAGCCTGGAGAAG(+CAGTGT)GATCCCTCCGGTTCCCATCTGTGCCTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605223833_u31u GGTCAGCTCCAGGCACAGATGGGAACCGGAGG(+CAGTGT)GATCCTTCTCCAGGCTCCTCAGTTTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605223831_h0yh ACCACATGGTGGCTCACAACCATCTGTAATAG(+CAGTGT)GATCCAATGCACTTTTCTGGTGTGTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605223830_fcit TGTCTTCAGACACACACCAGAAAAGTGCATTG(+CAGTGT)GATCCTATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605223827_k9jy ATCACACCACCATTTGTGCCTCCATCCCAGTG(+CAGTGT)GATCCAACACCAACCTCTGACCTCCACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605223826_5qwk GAAGTTCCTGTGGAGGTCAGAGGTTGGTGTTG(+CAGTGT)GATCCACTGGGATGGAGGCACAAATGGTGGT NGG 10 8 17 8 24 0 100 5 10