Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223809_3ksa TGAGCTTAGCTTGTGCTGGCGGCTGAACTCAG(+CAGTGT)GATCCTGTCCTGCACAGGCTAGGCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605223808_pl68 GGTAGAGGGCCTGCCTAGCCTGTGCAGGACAG(+CAGTGT)GATCCTGAGTTCAGCCGCCAGCACAAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605223806_zqtn TAATACCAACATTCACATACTGTATTACAATG(+CAGTGT)GTACCACAATATAAAATAAAATATGTATAAA NGG 10 8 17 8 24 0 100 5 10
    240605223805_9oph GTCAGTTTATACATATTTTATTTTATATTGTG(+CAGTGT)GTACCATTGTAATACAGTATGTGAATGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605223801_va1x CCCATAGGCACAAGGCGAGGGATGGTCCCAAG(+CAGTGT)GATCCGTAAGACAGTGTTTGCCATCCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605223800_8lun ACCAGACAGAGGATGGCAAACACTGTCTTACG(+CAGTGT)GATCCTTGGGACCATCCCTCGCCTTGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605223758_7yzc ACAAATCACAGCTCTGTGGTGTTAGGGCCGTG(+CAGTGT)GATCCTAAGCTGTGTGAACACAGAGAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605223757_3987 CTTTCCCTCCTCTCTGTGTTCACACAGCTTAG(+CAGTGT)GATCCACGGCCCTAACACCACAGAGCTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605223755_uuj1 TTTTACATGGGATACACACGTTAGCCCACCTG(+CAGTGT)GTACCCACCTGGAATATTTCCTAACTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605223753_qp4l TCAAATAACCAGTTAGGAAATATTCCAGGTGG(+CAGTGT)GTACCAGGTGGGCTAACGTGTGTATCCCATG NGG 10 8 17 8 24 0 100 5 10