Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223930_hcos TCCTCCACCAAGTACAATACATAGTATCTCAG(+CAGTGT)GATCCAGTGACACCTATACATCTTGGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605223929_v02s GGGACAGGGACCAAGATGTATAGGTGTCACTG(+CAGTGT)GATCCTGAGATACTATGTATTGTACTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605223928_ysjk GGTCCAAAGACTTGAGGGACTGTGTACTCAAG(+CAGTGT)GTACCAGGAATGGCTACGTCAGAACCCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605223926_vxyz TACAAAACAGGGTTCTGACGTAGCCATTCCTG(+CAGTGT)GTACCTTGAGTACACAGTCCCTCAAGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605223924_muk7 CTACCCTCATGCCCCCTTTGCCTTTCCCTCGG(+CAGTGT)GATCCTGTTTGGGCTCCCTTTCCCCAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605223923_5c4h AACACTTGCTTGGGGAAAGGGAGCCCAAACAG(+CAGTGT)GATCCCGAGGGAAAGGCAAAGGGGGCATGAG NGG 10 8 17 8 24 0 100 5 10
    240605223921_vvy4 AACTGCCATTCTCTGAAGACAGAGCAACAGGG(+CAGTGT)GTACCCCTGCCCCAGGGCTGCTCTCCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605223918_3sko GAGGGCCAAGGGAGAGCAGCCCTGGGGCAGGG(+CAGTGT)GTACCCCTGTTGCTCTGTCTTCAGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240605223917_mx78 CCAGAGAGCTCTGAGAGAGGACAAGGCAGCTG(+CAGTGT)GTACCCTGACCAATGTCCTCAGCCACACACA NGG 10 8 17 8 24 0 100 5 10
    240605223915_37jo GAGGGTGTGTGTGGCTGAGGACATTGGTCAGG(+CAGTGT)GTACCAGCTGCCTTGTCCTCTCTCAGAGCTC NGG 10 8 17 8 24 0 100 5 10