Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223959_5o8v TCTGGTTAGTATAAGTCAAATCAGGGTATTTG(+CAGTGT)GTACCTGTCTTGCTGTACTGAATTATAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605223958_fzgk ATTATTAGCTATAATTCAGTACAGCAAGACAG(+CAGTGT)GTACCAAATACCCTGATTTGACTTATACTAA NGG 10 8 17 8 24 0 100 5 10
    240605223956_37ab CACTGCCTGTGCTGCTAGGATTTAGTTATAAG(+CAGTGT)GATCCAGTACTCCAGGGCCAGGGCCAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605223955_swm5 CTCTGTACACTGGCCCTGGCCCTGGAGTACTG(+CAGTGT)GATCCTTATAACTAAATCCTAGCAGCACAGG NGG 10 8 17 8 24 0 100 5 10
    240605223953_xowh TGAATTGGGATTACTTTGATATTTTATTTTGG(+CAGTGT)GATCCTGTGCCACAAAATGACATGTGTAATG NGG 10 8 17 8 24 0 100 5 10
    240605223951_0zhq GTAAACATTACACATGTCATTTTGTGGCACAG(+CAGTGT)GATCCCAAAATAAAATATCAAAGTAATCCCA NGG 10 8 17 8 24 0 100 5 10
    240605223950_t7e7 GAGATACATAAGGCACAACAGCTTTCCTTCAG(+CAGTGT)GATCCTTCTCTCTGTCCTAGAGTCTTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605223949_ocqf CTACCGTCTGAAGACTCTAGGACAGAGAGAAG(+CAGTGT)GATCCTGAAGGAAAGCTGTTGTGCCTTATGT NGG 10 8 17 8 24 0 100 5 10
    240605223947_uvww CATCAACTGCAAACGTGAGGGGAGAAAGCATG(+CAGTGT)GTACCCAACCAAATTCCACATTTCAGCAATA NGG 10 8 17 8 24 0 100 5 10
    240605223946_cu4q TGAAGTATTGCTGAAATGTGGAATTTGGTTGG(+CAGTGT)GTACCATGCTTTCTCCCCTCACGTTTGCAGT NGG 10 8 17 8 24 0 100 5 10