Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224201_y2vr TGGATGCTTTCTGAGGGATGGTAATAGCTAAG(+CAGTGT)GATCCAGGACTGTGACTTAACCATTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605224200_7ao1 CTGGGGTGCAGTGTGAGGGTGGGCGCTTGTAG(+CAGTGT)GATCCCAAACTGGTGGGGGTGAGAAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605224158_0cq2 TTCCCTGCCCTTTCTCACCCCCACCAGTTTGG(+CAGTGT)GATCCTACAAGCGCCCACCCTCACACTGCAC NGG 10 8 17 8 24 0 100 5 10
    240605224157_dz8j ATCCTGCCCTCCCCAGCCAGCCCTCACAACTG(+CAGTGT)GATCCAATGCTAATTTGTCAAAAAAGGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605224155_3dmy AGACAATTGCCTTTTTTGACAAATTAGCATTG(+CAGTGT)GATCCAGTTGTGAGGGCTGGCTGGGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605224154_6yh2 CATTCCCTGCTCTCTGTTGACCTGTGTGGATG(+CAGTGT)GTACCAGCAGACTTCCAACCCTCCAGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605224153_0mpf TGACCAGAAGCTGGAGGGTTGGAAGTCTGCTG(+CAGTGT)GTACCATCCACACAGGTCAACAGAGAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605224151_xv7v GTTTCTTGACTGCATATGAATAAGCATCTGAG(+CAGTGT)GTACCAGAGAAAGGGGGTGCCTTTCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605224150_svlo AGGCTCTCTGGGAAAGGCACCCCCTTTCTCTG(+CAGTGT)GTACCTCAGATGCTTATTCATATGCAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605224148_edf2 GATCTCTGGCTTCCGTGACGCCGTCAATATTG(+CAGTGT)GATCCTCACCAGGACCCCTCCTGGTTTCCTG NGG 10 8 17 8 24 0 100 5 10