Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224435_hv36 AAAATACTTGAAATGTTTATTTGCTTTCTCAG(+CAGTGT)GATCCAAACTTTTCCTTGTTAGATAACAGCA NGG 10 8 17 8 24 0 100 5 10
    240605224433_u0g1 ACAGGAAGTTCCTTAAAAGCATATCATCTTAG(+CAGTGT)GTACCCTCTCTGAGGGTATGACTTCAAATTA NGG 10 8 17 8 24 0 100 5 10
    240605224430_cey0 TTAACTAATTTGAAGTCATACCCTCAGAGAGG(+CAGTGT)GTACCTAAGATGATATGCTTTTAAGGAACTT NGG 10 8 17 8 24 0 100 5 10
    240605224428_v64p ATGGAACTTTGGCCTCCTTTACGCTGGGAAAG(+CAGTGT)GATCCCACCACTGAGCTACAACCCAGCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605224426_90so GCTAGAAGGGCTGGGTTGTAGCTCAGTGGTGG(+CAGTGT)GATCCTTTCCCAGCGTAAAGGAGGCCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605224424_xczg CTTGTGGGTGTCTGGAGAATCCCTGGGCCAGG(+CAGTGT)GATCCCAGTGCTGCAGTGGGCCAGAAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605224422_d7qg GGGGGGAAGCTTCTGGCCCACTGCAGCACTGG(+CAGTGT)GATCCCTGGCCCAGGGATTCTCCAGACACCC NGG 10 8 17 8 24 0 100 5 10
    240605224421_b09z TTCCCCATTGGGAAGCATAAAGCACTTTTGTG(+CAGTGT)GTACCTTTCACTGGTAAATATTTATATCACA NGG 10 8 17 8 24 0 100 5 10
    240605224419_5e5s TTCAATGTGATATAAATATTTACCAGTGAAAG(+CAGTGT)GTACCACAAAAGTGCTTTATGCTTCCCAATG NGG 10 8 17 8 24 0 100 5 10
    240605224417_jsa4 TAAGACTGCTAGGGGATGGAAAGATCTGGGTG(+CAGTGT)GATCCATGTTTTCTCTGTTTGAAATACCTTT NGG 10 8 17 8 24 0 100 5 10