Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224543_v9cu AGGAAGACCCCGTTCATTCACCTAACCTTCTG(+CAGTGT)GTACCAAGGCTGTCAGTGCCAATGGATATGA NGG 10 8 17 8 24 0 100 5 10
    240605224541_y4sf ATGACAATGCAAGGTTCACCTCAGTGTTGAGG(+CAGTGT)GATCCAAGCTGAGGTGAAGCTGGGGACTATG NGG 10 8 17 8 24 0 100 5 10
    240605224540_5ylx ACATCCATAGTCCCCAGCTTCACCTCAGCTTG(+CAGTGT)GATCCCTCAACACTGAGGTGAACCTTGCATT NGG 10 8 17 8 24 0 100 5 10
    240605224538_zb2c TTTTAGGTTAGCTTCACTGTTGATATCCTTGG(+CAGTGT)GTACCTGCCTTCCAAATGGTGGGATTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605224536_91wi ACAGGCCTACAATCCCACCATTTGGAAGGCAG(+CAGTGT)GTACCCAAGGATATCAACAGTGAAGCTAACC NGG 10 8 17 8 24 0 100 5 10
    240605224534_kwcg CTATTCAGTAACCTCAGGCTCTGAGTCCCAGG(+CAGTGT)GATCCTGAGAGATCACTGCACGGGCCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605224532_vj62 GCTGCAGGAAGGCCCGTGCAGTGATCTCTCAG(+CAGTGT)GATCCCTGGGACTCAGAGCCTGAGGTTACTG NGG 10 8 17 8 24 0 100 5 10
    240605224531_e122 CTTCTTGGAGTTTGAAAAATGTGTTGATAATG(+CAGTGT)GATCCGCAGTAGCTTTCAGGGAGACGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605224530_9low GTCACTGACCCGTCTCCCTGAAAGCTACTGCG(+CAGTGT)GATCCATTATCAACACATTTTTCAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605224528_6zja GAGCCAAGCTTCCAGGTAGAATGGAGAATGTG(+CAGTGT)GATCCCAGTTGGACCCCTGGGCTTTCCACCT NGG 10 8 17 8 24 0 100 5 10