Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204614_n46e GCAGCAGAGTAACAAGGCAAGGGGGTAACATG(+CAGTGT)GTACCAATTAGCCTCGGAGCAGAGCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605204612_y9zq GTCTGCCGAGTTCCTATCATTTCCACTCCCAG(+CAGTGT)GATCCATGGTATTCAGATCAAGTAAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605204610_yzr5 CCTCCACAGCCTTACTTGATCTGAATACCATG(+CAGTGT)GATCCTGGGAGTGGAAATGATAGGAACTCGG NGG 10 8 17 8 24 0 100 5 10
    240605204608_do11 GCTTCTGCAAATTATCGAAGCTTCTGAGGAAG(+CAGTGT)GATCCATCCAGAGGCTCTGCTTGCCCGCCGC NGG 10 8 17 8 24 0 100 5 10
    240605204606_r1id CGCAGGCGGCGGGCAAGCAGAGCCTCTGGATG(+CAGTGT)GATCCTTCCTCAGAAGCTTCGATAATTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605204605_fsjc CCAGGCCTCCTGCATCCTGCCCAGACAGGCAG(+CAGTGT)GTACCTGTCTCCCAGTCACAGCTGGGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605204604_csye CACCTGCACACCCAGCTGTGACTGGGAGACAG(+CAGTGT)GTACCTGCCTGTCTGGGCAGGATGCAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605204602_wnjl CAGGCCCAAGACGTCCCAGTGTGCCCCAAATG(+CAGTGT)GATCCTACTTATTTCAAGTCAGCTGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605204601_8pfx TGCCCTGGGCTCAGCTGACTTGAAATAAGTAG(+CAGTGT)GATCCATTTGGGGCACACTGGGACGTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605204559_4ew6 GCAACTATTTCTAGGGCAGAGCAAAGGCCTGG(+CAGTGT)GATCCGGGATGAGCTTCAGGTTCTCCAGCTC NGG 10 8 17 8 24 0 100 5 10