Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204539_oidm AAAGGAAGGGTTTCCACTGAGGTACTTAGGAG(+CAGTGT)GATCCCACCTCTCCAGGTATCGTGACAACTG NGG 10 8 17 8 24 0 100 5 10
    240605204538_ae62 CATCTCCAGATACACAGTATGTCCAGTTGGTG(+CAGTGT)GTACCTGAGCCCACCCTTGACTAGGGCAGCG NGG 10 8 17 8 24 0 100 5 10
    240605204536_n8de CAGGCCGCTGCCCTAGTCAAGGGTGGGCTCAG(+CAGTGT)GTACCACCAACTGGACATACTGTGTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605204535_yy4j CCATGTGGGCTCCATGGATCAAACTAATGGTG(+CAGTGT)GTACCAATAAGCCATCTTGCTGGCCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605204534_gciv TTGAATTCTAGGGCCAGCAAGATGGCTTATTG(+CAGTGT)GTACCACCATTAGTTTGATCCATGGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605204533_a8b8 GCCTGGGCCATGGCCTCTTCTAAGAAAAGTTG(+CAGTGT)GTACCAACCACTAATGGTCCTTTTCTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605204531_qtxd CTAGAAGAGCAGAAAAGGACCATTAGTGGTTG(+CAGTGT)GTACCAACTTTTCTTAGAAGAGGCCATGGCC NGG 10 8 17 8 24 0 100 5 10
    240605204529_1oct TCCTGGCATCCTGCAGCCTTTAAGACCTGGGG(+CAGTGT)GATCCCTTACAGAAACAGTCTTAGCCTTTCG NGG 10 8 17 8 24 0 100 5 10
    240605204527_vdw7 AGGCTCGAAAGGCTAAGACTGTTTCTGTAAGG(+CAGTGT)GATCCCCCAGGTCTTAAAGGCTGCAGGATGC NGG 10 8 17 8 24 0 100 5 10
    240605204526_1l87 ACTGCATTGGTAGGGTACAAAGGTGTTCAGAG(+CAGTGT)GATCCCAGTCTGAGTGATTAGCCTAAGACTG NGG 10 8 17 8 24 0 100 5 10