Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204428_8i8w TTTGAAGTTCAAGTTCTCTCTCCCAACCATGG(+CAGTGT)GTACCATTCAGGCTTGTTCTCAACCTAGATG NGG 10 8 17 8 24 0 100 5 10
    240605204427_1qyj ACCCAGGTAATGTGGGCGTGCTCAGAGCGTCG(+CAGTGT)GATCCGGTGGCTGCTTTACACCTGGTGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605204425_0tpb TGCTGTTCACACCAGGTGTAAAGCAGCCACCG(+CAGTGT)GATCCGACGCTCTGAGCACGCCCACATTACC NGG 10 8 17 8 24 0 100 5 10
    240605204423_bv62 CCGAGCCAAGTGTCAGATGCACCCTCCACTGG(+CAGTGT)GATCCTCCCCTGAGGATCTAAGGTGTGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605204421_gs2c GTTTCTGCACACACCTTAGATCCTCAGGGGAG(+CAGTGT)GATCCCAGTGGAGGGTGCATCTGACACTTGG NGG 10 8 17 8 24 0 100 5 10
    240605204418_dhi2 TCGCGGTGGCCAGGCCCTTGCTCACTCCCTGG(+CAGTGT)GATCCCGATTGAGTTGTCCACCTCTCACTGT NGG 10 8 17 8 24 0 100 5 10
    240605204417_bth9 AGGAGACAGTGAGAGGTGGACAACTCAATCGG(+CAGTGT)GATCCCAGGGAGTGAGCAAGGGCCTGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605204415_viko GAGAAATGGGGGCACTGCACCCCCACTTCCTG(+CAGTGT)GATCCTGACTCTTAGGCTGGTGGCCCTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605204413_ovo6 GGCGGGGCAAGGGCCACCAGCCTAAGAGTCAG(+CAGTGT)GATCCAGGAAGTGGGGGTGCAGTGCCCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605204411_b27v ACGTACATTGAGCTCCAGAAGGAAAAGAGAGG(+CAGTGT)GATCCTAACCAAGGGCCACCAGGAAGCAGCA NGG 10 8 17 8 24 0 100 5 10