Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204410_pc4v TTGCTTGCTGCTTCCTGGTGGCCCTTGGTTAG(+CAGTGT)GATCCCTCTCTTTTCCTTCTGGAGCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240605204409_y8f3 CAGGAGTCCCTGAGGTTCCCAGCTGCTGTCAG(+CAGTGT)GATCCTGTGAGTTGGGGCAAGCAGGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605204407_t98s GGTGGCAGCATCCTGCTTGCCCCAACTCACAG(+CAGTGT)GATCCTGACAGCAGCTGGGAACCTCAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605204406_mklx CTGTCACATGCAGCAGACAGGCAGCATTAAGG(+CAGTGT)GTACCCTAGCTCTGTTAGGGCAGAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605204404_kyv6 ACATTCCCAGGCTCTGCCCTAACAGAGCTAGG(+CAGTGT)GTACCCTTAATGCTGCCTGTCTGCTGCATGT NGG 10 8 17 8 24 0 100 5 10
    240605204402_x40o GGCGGCACTCGCCCCTCCTGGGGATACAGCAG(+CAGTGT)GATCCGGCCTCAAGAGGCTTCAGGTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605204400_a5wl GAGTGTCAAGCACCTGAAGCCTCTTGAGGCCG(+CAGTGT)GATCCTGCTGTATCCCCAGGAGGGGCGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605204358_bsec GGTGTAGCCCTACCTACAGTGTGACTAGGCCG(+CAGTGT)GTACCGATCCTGCCTGGTTCTAACCAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605204357_o3lq GTCTGTTTTCTGGTTAGAACCAGGCAGGATCG(+CAGTGT)GTACCGGCCTAGTCACACTGTAGGTAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605204356_wlko AACCCCTTTCAACAGCCCTCCCTCTTCAGTAG(+CAGTGT)GTACCCGCCCCTAGTGATGATGGGCAGACAG NGG 10 8 17 8 24 0 100 5 10