Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204524_nhbf TGCTTCAGTCTTAGGCTAATCACTCAGACTGG(+CAGTGT)GATCCTCTGAACACCTTTGTACCCTACCAAT NGG 10 8 17 8 24 0 100 5 10
    240605204522_z0ci CTCCCACGACCTCGGTCCCAGTTGGACACCAG(+CAGTGT)GTACCTCTGGAAAATGAGACAACCGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605204520_ja9e CACGCACAGCCCGGTTGTCTCATTTTCCAGAG(+CAGTGT)GTACCTGGTGTCCAACTGGGACCGAGGTCGT NGG 10 8 17 8 24 0 100 5 10
    240605204518_ot36 GGTGGAAGGTAGTCGGATCTTCCAGAAGGCTG(+CAGTGT)GATCCTCACGAGGTTGATCAACCTGTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605204517_d5m4 CTATCTTTAGACAGGTTGATCAACCTCGTGAG(+CAGTGT)GATCCAGCCTTCTGGAAGATCCGACTACCTT NGG 10 8 17 8 24 0 100 5 10
    240605204515_cxxm GCTGGGATCATGGATAAGTGTTAACATGCCAG(+CAGTGT)GATCCTCTTCTATCTTTAAGTCTAAGATAGT NGG 10 8 17 8 24 0 100 5 10
    240605204513_79px AAGTCACTATCTTAGACTTAAAGATAGAAGAG(+CAGTGT)GATCCTGGCATGTTAACACTTATCCATGATC NGG 10 8 17 8 24 0 100 5 10
    240605204511_dov8 TGCTGGACCTGGGCAGGATGTACTCCCCTGGG(+CAGTGT)GTACCGGGGCCTGCCTGCGGCTTACCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605204508_ugfp TGCAGCAGAAGGTAAGCCGCAGGCAGGCCCCG(+CAGTGT)GTACCCCAGGGGAGTACATCCTGCCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605204507_pf6e TTGGGAGCAGTTGTGGAACAGAGGACAGATCG(+CAGTGT)GTACCATACAGAGGCATGGGTTCGCTGTAGC NGG 10 8 17 8 24 0 100 5 10