Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204557_tz97 CACAGGAGCTGGAGAACCTGAAGCTCATCCCG(+CAGTGT)GATCCCAGGCCTTTGCTCTGCCCTAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240605204554_uqj7 GTGTCAATCACAGGGTTCTGACAGAAACCTGG(+CAGTGT)GATCCCTTTGAAGGGCAGGCAGCCGCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605204552_7512 GCGGAGCCTTGCGGCTGCCTGCCCTTCAAAGG(+CAGTGT)GATCCCAGGTTTCTGTCAGAACCCTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240605204550_63r7 TGGAGGCATATCAGAACCACAGTTAGGGAAGG(+CAGTGT)GATCCAAGTTGGTTGGAGAACAGCTAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605204549_3qub CACCCAGTCCTAGCTGTTCTCCAACCAACTTG(+CAGTGT)GATCCCTTCCCTAACTGTGGTTCTGATATGC NGG 10 8 17 8 24 0 100 5 10
    240605204546_69zj AGGGCTTTGTCCCCTTTCTGTAAGTCTCTTGG(+CAGTGT)GATCCTGTGTAGGAGCTGTTCTCATTAAACA NGG 10 8 17 8 24 0 100 5 10
    240605204545_6jiq GTTGATGTTTAATGAGAACAGCTCCTACACAG(+CAGTGT)GATCCCAAGAGACTTACAGAAAGGGGACAAA NGG 10 8 17 8 24 0 100 5 10
    240605204544_1tj3 GGCCATAGCTGAGCAGAGAAGGTTCAATGCAG(+CAGTGT)GTACCCATCTGATGGGTTAAGATGGATGTAA NGG 10 8 17 8 24 0 100 5 10
    240605204542_o86h TAGTATTACATCCATCTTAACCCATCAGATGG(+CAGTGT)GTACCTGCATTGAACCTTCTCTGCTCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605204540_5j8i CACACCAGTTGTCACGATACCTGGAGAGGTGG(+CAGTGT)GATCCTCCTAAGTACCTCAGTGGAAACCCTT NGG 10 8 17 8 24 0 100 5 10