Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210037_1e4p GAGCTCTGTACTGTGCAGAGGAAGTGGCCAGG(+CAGTGT)GTACCAACGAGGGAGGAGCGCTCTGTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605210034_075o AGTCAGTAAGAATGACCCTGCTGTGCCCCAGG(+CAGTGT)GTACCCATTCCCCACAGCATGCTTATGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605210033_ko1v GTCTCATGGCATAAGCATGCTGTGGGGAATGG(+CAGTGT)GTACCCTGGGGCACAGCAGGGTCATTCTTAC NGG 10 8 17 8 24 0 100 5 10
    240605210031_54uc CTTGCATGTGGAGACCAGGGGTTGACATCAAG(+CAGTGT)GATCCTCCTTAATCATTTATCCACTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605210029_fs1q TTTAAATGGTGAGTGGATAAATGATTAAGGAG(+CAGTGT)GATCCTTGATGTCAACCCCTGGTCTCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605210026_08hl GGCACTGACCTGCCCCATTTCCTGATCCTTGG(+CAGTGT)GATCCTAGAAACGCATACAAGGAGGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240605210025_jgg8 AGGCCACTGTCCCTCCTTGTATGCGTTTCTAG(+CAGTGT)GATCCCAAGGATCAGGAAATGGGGCAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605210024_7zzg TCCTGTTCAAATGTACCTCAGTGGAGAGAGAG(+CAGTGT)GATCCTCGCTTCTACACTGTGTGGCCTGACG NGG 10 8 17 8 24 0 100 5 10
    240605210023_styl ACTTCCGTCAGGCCACACAGTGTAGAAGCGAG(+CAGTGT)GATCCTCTCTCTCCACTGAGGTACATTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605210021_69y6 CCTGTAGAAGGCAAATGAGACACTGATTAGTG(+CAGTGT)GATCCCTGCAGAACTGGGTCCTAGATCCAAA NGG 10 8 17 8 24 0 100 5 10