Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204245_42ke CCAGGTGGCACTGGGATGCCAGTTGGCAGCTG(+CAGTGT)GATCCTTCTGTTCACACGACTTGGAACCTTT NGG 10 8 17 8 24 0 100 5 10
    240605204243_e8qe TGCTTCCTACTTACTATAACAGAAGAGTTTGG(+CAGTGT)GTACCCCCTCCTCATGCACCCAAGAAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605204241_dkwf GGGAACCTCCTTCTTGGGTGCATGAGGAGGGG(+CAGTGT)GTACCCAAACTCTTCTGTTATAGTAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605204240_emg3 ATGGTGCAGGAGAGGAGCTGAGAGTTCTACTG(+CAGTGT)GATCCTCAGAGAGTAGGAAGAGTGACACTAG NGG 10 8 17 8 24 0 100 5 10
    240605204239_tfk3 CAGGCCTAGTGTCACTCTTCCTACTCTCTGAG(+CAGTGT)GATCCAGTAGAACTCTCAGCTCCTCTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605204237_9erl GCGGATCTCCAGCCTGGAATCAGAAGTGTGAG(+CAGTGT)GTACCGGCAGCTCTGGTTCCTGCTGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240605204236_vefb AGAATCCAGTCCAGCAGGAACCAGAGCTGCCG(+CAGTGT)GTACCTCACACTTCTGATTCCAGGCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605204235_0mq5 GTGTGGGGGTGATGAGGGCAGCTTTTCAAGTG(+CAGTGT)GTACCCCGCAATTCAGGGGGTCCCTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605204233_0re3 CTTTGGAGCCCAGGGACCCCCTGAATTGCGGG(+CAGTGT)GTACCACTTGAAAAGCTGCCCTCATCACCCC NGG 10 8 17 8 24 0 100 5 10
    240605204231_lqwx CATCCCCACTTATAGCCAATGTACCTTGGAAG(+CAGTGT)GTACCAGGCAGGCTGCTTCAGGGATGCTGTG NGG 10 8 17 8 24 0 100 5 10